Nanostrip and Lumibead Assays
The advantage...
The high binding capacity combined with the low autofluorescence of rHEALTH hydrogel microparticles allow for superior analytical sensitivity.
Right Figure. Spiked TNFa samples were analyzed via conventional polystyrene beads, ELISA, and Nanostrips using the same antibody pair. The assays were performed in a similiar manner, except for technology platform.
The results are demonstrated in a 3-way comparison of Nanostrips with ELISA and bead-based assays in the figure to the right. Using the same antibody pair, the nanostrips have the best senstivity at < 0.1 pg/mL compared with chemifluorescent ELISA at 10 pg/mL and other conventional polystyrene bead-based assays at 10s of pg/mL.
![Results are plotted with arbitrary units (AU) versus [TNFa] (pg/mL). Open symbols indicate the LOD. LOD for Nanostrips is < 0.1 pg/mL for Nanostrips as compared to 10 pg/mL for ELISA and 80 pg/mL for conventional beads. ](https://images.squarespace-cdn.com/content/v1/582c9cd19de4bbb2b259cf0d/1480946845369-QDOC1AUW6HB6VWTY1AAV/image-asset.png)
Results are plotted with arbitrary units (AU) versus [TNFa] (pg/mL). Open symbols indicate the LOD. LOD for Nanostrips is < 0.1 pg/mL for Nanostrips as compared to 10 pg/mL for ELISA and 80 pg/mL for conventional beads.
A wide variety of assay formats are possible including: (A) sandwich immunoassay, (B) competitive immunoassay, and (C) nucleic acid detection. Other assay formats are possible as well.
Our particles are well-suited for immunoassays, nucleic acids, and other types of molecular assays that are conventionaly performed in various laboratory settings. Nanostrips are capable of exceptionally high levels of multiplexing based on unique 1D barcodes implemented in the blue fluorescence channel. LumiBeads are utilized when high-sensitivity is desired along with moderate levels of multiplexing.
Capabilities of Nanostrips and LumiBeads compared with conventional assay approaches.